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宿主细胞残留DNA检测试剂盒

宿主细胞残留DNA检测试剂盒

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商品描述
Host Cell DNA Residues: Recombinant protein drugs, antibody drugs, vaccines and other products in biological products are expressed by continuous passage of animal cell lines. Although strictly purified, DNA fragments of host cells may still remain in the product. These residual DNA may introduce infectious or tumorigenic risks, such as residual DNA may carry the HIV virus or Ras oncogene. Therefore, WHO and national drug regulators generally only allow the presence of residual DNA in biologics at doses below 100 pg / dose. Depending on the source of impurities and the process, special conditions up to 10ng / dose

Name: Residual Host Cell DNA kits (Each product name can be clicked to enter detailed product information)

Item No.

Name of the product

Target Species

D555T

CHO DNA Amplification in tubes

Mammalian

D555W

CHO DNA Amplification in wells

Mammalian

D550T

CHO Host Cell DNA in tubes

Mammalian

D550W

CHO Host Cell DNA in tubes

Mammalian

D100T

DNA Extraction Kit in Tubes

ALL

D100W

DNA Extraction Kit in Wells

ALL

D415T

E.coli DNA Amplification in tubes

E.coli

D415W

E.coli DNA Amplification in wells

E.coli

D410T

E.coli DNA Amplification in wells

E.coli

D410W

E.coli Host Cell DNA in wells

E.coli 


product manual:
DNA extraction kits: These kits are able to isolate <1pg / mL levels of residual DNA. The 96-well well format increases efficiency by increasing throughput while minimizing mistakes that can occur when handling individual tubes. The superior co-precipitating agent keeps pellets seated onto the wells an does not interfere with qPCR. The proprietary reagents in the kit do not use iodine or glycogen so your clothes will be stain free! Samples are available in less than 90 minutes and can be in dye binding assays, qPCR, and Threshold® .

Residual Host Cell DNA kits: These DNA dye binding kits untiize PicoGreen® and come complete with DNA extraction reagents and cell line specific DNA calibrators. 96 individual extractions can be performed in less than 2 hours. The kits have been optimized for sensitivity to 400 pg / mL.

PCR kits: These kits contain DNA extraction reagents, DNA calibrators and validated specific primers compatible with your PCR system. These amplification based kits have a sensitivity of 1 part per billion.

Threshold® System is a trademark of Molecular Devices, Inc.

Quant-iT ™ PicoGreen® dsDNA Reagents trademarks of Life Technologies Corporation.
This transfer is this product is contingent on the buyer using the purchased product solely in industrial quality control testing for testing pharmaceutical manufacturing processes, food manufacturing processes, bioprocessing, biofermentation and probiotic production .