Nucleic acid purification: nucleic acid extraction and purification is the basis of molecular biology experiments, nucleic acid purification method is the most important factor affecting the quality of extracted nucleic acids, but also the key to the success of downstream molecular biology experiments. At present, common nucleic acid purification methods include PC extraction / alcohol precipitation, high salt precipitation protein / alcohol precipitation, spin column and biological magnetic beads. Nucleic acid purification can be divided into plasmid extraction, gel recovery and PCR purification of three parts.

Name: Monarch ™ Plasmid Miniprep Kit

Product Manual

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The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. 

Monarch Plasmid Miniprep Column Design



Advantages: 

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• Elute in as little as 30 μl
• Prevent buffer retention and salt carry-over with optimized column design
• Reduce hands on time with faster protocols and less spin time
• Monitor completion of certain steps using colored buffer system
• No need to add RNase before starting
• Easily label columns using tab and frosted surfaces
• Buffers and columns available separately
• Significantly less plastic used when compared with other kits
• Responsibly-sourced and recyclable packaging
• No hazardous materials fees 

Specifications

Culture Volume:	1–5 ml
Binding Capacity:	up to 20 μg
Plasmid Size:	up to 25 kb
Typical Recovery:	up to 20 μg. Yield depends on plasmid copy number, host strain, culture volume, and growth conditions.
Elution Volume:	≥ 30 μl
Purity:	A260/280 and A260/230 ≥ 1.8
Protocol Time:	10½ minutes of spin and incubation time
Compatible Downstream Applications:	restriction digestion and other enzymatic manipulations, transformation, transfection, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc.